The cloche (clom39) and spadetail (sptb104) mutations affect definitive progenitors in the dorsal aorta. Whole-mount in situ hybridization of 48-h-old wild-type (A, D), clom39 (B, E), and sptb104 (C) embryos with c-myb (A–C) and flk1 (D, E). All three mutations greatly reduce the number of cells that express c-myb in the dorsal aortas of 48-h-old embryos (arrows A–C). Cells expressing flk1 are seen in the tails of 48-h-old embryos (arrows D, E).
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