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Fig. 6 shh expression in flh and cyc;flh mutants during early segmentation. Dorsal views (anterior to the top) of whole-mount RNA in situ hybridization at the 3-somite stage (11 hr) with an antisense probe against shh in blue. shh expression in wild types is present in the forming notochord and floor plate (A). In flh mutants, shh expression is patchy in what will be the trunk region, reflecting the missing notochord and the discontinuous floor plate (B). In cyc;flh mutants, shh expression is completely undetected in the trunk (C) with the only detectable expression in the tail bud (arrow).
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Reprinted from Developmental Biology, 187(2), Beattie, C.E., Hatta, K., Halpern, M.E., Liu, H., Eisen, J.S., and Kimmel, C.B., Temporal separation in the specification of primary and secondary motoneurons in zebrafish, 171-182, Copyright (1997) with permission from Elsevier. Full text @ Dev. Biol.