PUBLICATION
Temporal separation in the specification of primary and secondary motoneurons in zebrafish
- Authors
- Beattie, C.E., Hatta, K., Halpern, M.E., Liu, H., Eisen, J.S., and Kimmel, C.B.
- ID
- ZDB-PUB-970812-3
- Date
- 1997
- Source
- Developmental Biology 187(2): 171-182 (Journal)
- Registered Authors
- Beattie, Christine, Eisen, Judith S., Halpern, Marnie E., Hatta, Kohei, Kimmel, Charles B.
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- DNA-Binding Proteins
- Embryonic Development
- Embryonic Induction*
- Gastrula
- Hedgehog Proteins
- Homeodomain Proteins/genetics
- Immunohistochemistry
- In Situ Hybridization
- Mesoderm
- Motor Neurons*
- Mutation
- Plant Proteins/genetics
- Proteins/genetics*
- Spinal Cord/embryology*
- Trans-Activators*
- Transcription Factors/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins*
- PubMed
- 9242415 Full text @ Dev. Biol.
Citation
Beattie, C.E., Hatta, K., Halpern, M.E., Liu, H., Eisen, J.S., and Kimmel, C.B. (1997) Temporal separation in the specification of primary and secondary motoneurons in zebrafish. Developmental Biology. 187(2):171-182.
Abstract
In zebrafish there are two populations of motoneurons, primary and secondary, that are temporally separate in their development. To determine if midline cells play a role in the specification of these neurons, we analyzed both secondary and primary motoneurons in mutants lacking floor plate, notochord, or both floor plate and notochord. Our data show that the specification of secondary motoneurons, those most similar to motoneurons in birds and mammals, depends on the presence of either a differentiated floor plate or notochord. In the absence of both of these structures, secondary motoneurons fail to form. In contrast, primary motoneurons, early developing motoneurons found in fish and amphibians, can develop in the absence of both floor plate and notochord. A spatial correspondence is found between secondary motoneurons and sonic hedgehog-expressing floor plate and notochord. In contrast, primary motoneuronal specification depends on the presence of sonic hedgehog in gastrula axial mesoderm, the tissue that will give rise to the notochord. These results suggest that both primary and secondary motoneurons are specified by signals from midline tissues, but at very different stages of embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping