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Fig. S1 Cells with impaired motility due to Lbx2 knockdown still express myod.lbx2-MO and rhodamine (red) injected cells were transplanted into the margin, 75 degrees ventral to the shield, at shield stage. When control cells are transplanted into control embryos, the cells become distributed along the rostral caudal axis (A) and transplanted cells adjacent to the notochord express myod (A inset, arrow, ctrl > ctrl: n = 4). In contrast, transplanted lbx2-MO injected cells stay clumped and do not distribute along the rostral caudal axis, although cells adjacent to the notochord express myod normally (B inset, arrow, lbx2-MO > ctrl: n = 4/4). This effect on migration appears to be cell-autonomous because control cells transplanted into lbx1b-MO injected embryos behave normally (C, ctrl > lbx2-MO: n = 2). lbx2-MO + lbx2-mRNA injected cells become distributed along the rostral caudal axis (D, n = 13/15 rescued). Arrows indicate myod expressing transplanted cells. N: notochord. (A-D) Whole-mount embryos, dorsal views, rostral toward the top. Scale bar: 200 μm.