Fig. S2

Fig. S2 Reporter assay validating the functional binding of ergMO to its target sequence. (A) Shows EGFP fluorescence in EGFP-only (control) and Target:EGFP (test) groups in the absence of erg ATG-targeting morpholino. (B) erg ATG-targeting morpholino efficiently knocks down EGFP expression in Target:EGFP but not EGFP-only injected embryos. Uninjected controls are shown for each experiment and wavelength. Rhodamine dextran dye was used a tracer for morpholino injection. Panels show pools of randomly selected embryos. Methodological details are in text. Top panels: bright field (BF); central panels: Rhodamine fluorescence; lower panels: EGFP fluorescence. Bright-field and fluorescent images were taken with 8 ms and 8 s exposure times, respectively.