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Fig. S4 The ET11–9–Positive Segment Marks the Proximal-Most Extent of Multiciliated Cells
Two-dpf ET11–9 embryos were stained with anti-acetylated tubulin (A; red) and anti-GFP (B; green). (C) Merge of (A) and (B). (D) Close-up of the anterior (proximal) end of the ET11–9 GFP-positive domain showing compressed, acetylated tubulin-positive bundles of cilia in the lumen of GFP-positive tubule cells. Dorsal acetylated tubulin-positive cells are neurons. Arrows mark the most proximal bundles of cilia originating from pronephric multiciliated cells [23].
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