ZFIN Image: Davis et al., 2009, Fig. 5

Image

Figure Caption

Fig. 5 Motility and Departure of Infected Granuloma Macrophages

(A) Average speeds of infected and uninfected cells versus their bacterial volumes.

(B) Tracks of departing infected macrophages from Movie S11. Scale bar, 50 μm.

(C) Bacterial volumes and average speeds of departing macrophages in (B), compared to those that did not depart. p by Student's t test.

(D–F) Departure of infected macrophages from brain granuloma. (D) Granuloma immediately after photoactivation (red) and (E) 24 hr later, demonstrating granuloma growth and departure of infected macrophages (arrowheads). (F) DIC/red fluorescence overlay of departing macrophages (arrowheads). Dotted line represents granuloma edge. Scale bars in (D)-(F), 20 μm.

(G–I) Hematogenous dissemination from tail granuloma. (G) A single granuloma (arrow) photoactivated at 3 dpi. Scale bar, 300 μm. (H) At 4 dpi, photoactivated bacteria (arrowheads) seen in gill vasculature. (I) DIC image of cluster #2 from (H). Dashed line: limits of vasculature, e: erythrocyte, m: muscle. Scale bar, 10 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 136(1), Davis, J.M., and Ramakrishnan, L., The role of the granuloma in expansion and dissemination of early tuberculous infection, 37-49, Copyright (2009) with permission from Elsevier. Full text @ Cell