Fig. 5

Fig. 5 Temporal expression of ZF-RNases-1, -2 and -3 genes in adult zebrafish. A, 4-month-old zebrafish dissected into anterior (A), mid (M) and posterior (P) part toward the body plan axis and analyzed by reverse transcriptase followed by PCR (RT-PCR) for the expression of ZF-RNases-1, -2 and -3 genes. The -RT lane is a negative control for the reverse transcription reaction. RNAs ZF-Odc is a loading control. B, quantitative analysis of ZF-RNases-1, -2 and -3 genes performed by real-time PCR. ZF-RNases-1, -2 and -3 genes expression is mainly detected in the anterior (A) and mid- (M) parts of the body. Note, only a slight expression of ZF-RNases-1, -2 and -3 genes is observed in the posterior part (P). Quantified mRNA values were normalized by the amounts of ZF-Odc mRNA, the house keeping gene, and results are given as fold induction.