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Fig. S6
Morpholino knockdown efficacy of Gadd45 family members
Knockdown efficiencies of morpholinos against Gadd45α (4pg) (A), Gadd45αlike (4pg) (B), Gadd45β (2pg) (C), and Gadd45γ (2pg) (D) was assessed at 80% epiboly in cDNA made from total RNA obtained from wild type embryos or ones injected with respective morpholinos. Spliced and unspliced DNA length is shown. 28S (A), Gadd45γ (B-C) and Gadd45β (D) were used as a loading controls. Panels A, B, and D contain lanes cut and pasted next to each other from the same gel.
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Reprinted from Cell, 135(7), Rai, K., Huggins, I.J., James, S.R., Karpf, A.R., Jones, D.A., and Cairns, B.R., DNA demethylation in zebrafish involves the coupling of a deaminase, a glycosylase, and gadd45, 1201-1212, Copyright (2008) with permission from Elsevier. Full text @ Cell