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Fig. 4 Derivation of the stable transgenic TG(UAS-SV40:Luciferase) zebrafish reporter line.A. Potential F0 founder fish were identified by PCR as outlined in the Methods section. Two founders (ID1 and ID2) were identified. B. Expression of the Luciferase reporter in stable transgenic embryos. TG(UAS-SV40:Luciferase) F1 carriers were out-crossed and their offspring assayed for luciferase activity. Four F1 carriers derived from the ID1 founder (ID1.1, ID1.2, ID1.3 and ID 1.4) produced embryos with robust luciferase activity, while 3 carriers derived from the ID2 founder (ID2.1, ID2.2 and ID2.3) produced embryos lacking luciferase activity.