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Fig. 2 (A) Zebrafish embryos were infected at the 500- to 1000-cell stage with pseudotyped retroviral vector via injection of virions into four separate sites. 24 h after the infection, embryos were examined in the fluorescence microscope for GFP expression. This field is a typical view of the expression obtained. (B) Follicles from an Ef1-α-39-derived adult that was sacrificed to examine the ovaries. Note that the GFP is present in the nucleus (similar observations were made on follicles from a RVEG-19 female).
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Reprinted from Developmental Biology, 213(1), Linney, E., Hardison, N.L., Lonze, B.E., Lyons, S., and DiNapoli, L., Transgene expression in zebrafish: a comparison of retroviral-vector and DNA-injection approaches, 207-216, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.