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Fig. S6 RA treatments during late larval stages cause fusions of vertebral bodies. Alizarin Red (alR) stainings of vertebrae at 9 dpf (A,B) or 18 dpf (C-F). (A,C,E) Untreated siblings; numbers indicate anterior-posterior positions of shown vertebrae along the vertebral column. (B,D,E) Corresponding regions of animals treated with RA from 6-9 dpf (B), or from 15-18 dpf (D,F). RA treatments at these late developmental stages cause widespread fusions of centra, including centra 3-6, which ossify first and which have become insensitive to Bmp2 at 4 dpf (compare with Fig. 7). c, centrum; ha, hemal arch; na, neural arch.