PUBLICATION
Restriction of retinoic acid activity by Cyp26b1 is required for proper timing and patterning of osteogenesis during zebrafish development
- Authors
- Laue, K., Jänicke, M., Plaster, N., Sonntag, C., and Hammerschmidt, M.
- ID
- ZDB-PUB-081022-35
- Date
- 2008
- Source
- Development (Cambridge, England) 135(22): 3775-3787 (Journal)
- Registered Authors
- Hammerschmidt, Matthias, Laue, Kathrin, Plaster, Nikki, Sonntag, Carmen
- Keywords
- Cyp26b1, Retinoic acid, Bmp2, Cartilage, Bone, Chondrocyte, Osteoblast, Osteopontin, Osterix, Craniofacial development, Vertebra, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Base Sequence
- Body Patterning*
- Bone Morphogenetic Protein 2/genetics
- Bone Morphogenetic Protein 2/metabolism
- Branchial Region/embryology
- Branchial Region/enzymology
- Cytochrome P-450 Enzyme System/genetics
- Cytochrome P-450 Enzyme System/metabolism*
- Gene Expression Regulation, Developmental*
- Gene Expression Regulation, Enzymologic
- Mice
- Mutation/genetics
- Osteoblasts/enzymology
- Osteogenesis*
- Osteopontin/metabolism
- Phenotype
- Tretinoin/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 18927157 Full text @ Development
Citation
Laue, K., Jänicke, M., Plaster, N., Sonntag, C., and Hammerschmidt, M. (2008) Restriction of retinoic acid activity by Cyp26b1 is required for proper timing and patterning of osteogenesis during zebrafish development. Development (Cambridge, England). 135(22):3775-3787.
Abstract
Skeletal syndromes are among the most common birth defects. Vertebrate skeletogenesis involves two major cell types: cartilage-forming chondrocytes and bone-forming osteoblasts. In vitro, both are under the control of retinoic acid (RA), but its exact in vivo effects remained elusive. Here, based on the positional cloning of the dolphin mutation, we have studied the role of the RA-oxidizing enzyme Cyp26b1 during cartilage and bone development in zebrafish. cyp26b1 is expressed in condensing chondrocytes as well as in osteoblasts and their precursors. cyp26b1 mutants and RA-treated wild-type fish display a reduction in midline cartilage and the hyperossification of facial and axial bones, leading to fusions of vertebral primordia, a defect not previously described in the context of RA signaling. Fusions of cervical vertebrae were also obtained by treating mouse fetuses with the specific Cyp26 inhibitor R115866. Together with data on the expression of osteoblast markers, our results indicate that temporal and spatial restriction of RA signaling by Cyp26 enzymes is required to attenuate osteoblast maturation and/or activity in vivo. cyp26b1 mutants may serve as a model to study the etiology of human vertebral disorders such as Klippel-Feil anomaly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping