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Fig. S2

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ZDB-IMAGE-081103-37
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Source
Figures for Leung et al., 2008
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Fig. S2 Phylogram and RT-PCR analysis of the zebrafish gpr161. (A) Phylogram analysis revealed zebrafish Gpr161 protein is highly similar to the human counterpart within the purine receptor cluster branch of the δ-group of RHODOPSIN receptors. The protein sequences were aligned using T-coffee (http://igs-server.cnrs-mrs.fr/Tcoffee/tcoffee_cgi/index.cgi) and the phylogenetic tree was constructed using the Treetop program with a bootstrap value of 100and the phylogenetic tree was constructed using the Treetop program with a bootstrap value of 100 (http://www.genebee.msu.su/services/phtree_reduced.html). Genbank accession numbers for human C3AR (NP_004045), human GPR103 (NP_937822), humanTRHR (NP_003292), human P2RY11 (NM_002566), human GPR82 (NP_543007), human GPR18 (CAI14306), human EBI2 (P32249), human PTAFR (NP_000943), human GPR101 (NP_473362), human GPR161 (CAI22623), human RGR (AAI01111) and zebrafish Gpr161 (EU090912).(B) RT-PCR detection of the maternal zebrafish gpr161 transcript at 1 cell stage and zygotic transcript at sphere stage (4 hpf) onward to 96 hpf, for 26 and 30 PCR cycles. actin RT-PCR was used as RNA loading control, for 22 PCR cycles. (C) gpr161 expression in 1 dpf to 3 dpf zebrafish embryos. (D) Negative control of using sense probe of gpr161. All scale bars were 100 μm.

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Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.