Fig. 5 Activation of loxP-blocked reporter gene expression. A,B: A 48 hours postfertilization (hpf) embryo from a cross between a female Tg(zp3:cre, krt8:rfp) and male Tg(EF:loxP-mCherry-loxP-egfp) to show mCherry (A) and enhanced green fluorescent protein (EGFP; B) expression. C,D: A 48-hpf embryo from a cross between a female wild-type fish and male Tg(EF:loxP-mCherry-loxP-egfp) to show mCherry (C) and GFP (D) expression. E,F: View of mCherry (E) and GFP (F) expression in the ovary from a 1.5-month-old female double transgenic fish Tg(Zp3:Cre, Krt8:RFP)/ Tg(EF:loxp-mCherry-loxp-egfp. G,H: View of mCherry (G) and GFP (H) expression in the ovary from a 1.5-month-old female Tg(EF:loxp-mCherry-loxp-egfp fish. Scale bars = 100 μm.
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