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Fig. 8 Ectopic expression of pax2a rescues intermediate mesoderm patterning in osr1 morphants. (A-C) Expression of ae2 in the pronephros of 24 hpf control (A), osr1 morphant (B) and osr1 morphant co-injected with pax2a mRNA (C). Horizontal lines indicate the length of the wild-type ae2-positive proximal nephron segment. (D-F) Expression of scl in the anterior lateral mesoderm of 24 hpf control (D), osr1 morphant (E) and osr1 morphant co-injected with pax2a mRNA (F). Horizontal lines indicate the length of the wild-type scl-positive anterior lateral mesoderm. (G) Quantification of pax2a rescue of ae2-positive proximal pronephric nephron cells (see text for details).