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Fig. 5 Early-born trigeminal sensory neurons contribute to various subpopulations independently of late-born neurons. Subpopulation markers were analyzed in embryos lacking late-born trigeminal sensory neurons. (A-H) Embryos were incubated from 24 hpf to 72 hpf with 2% DMSO alone (A,C,E,G) or 20 mM hydroxyurea and 150 μM aphidicolin (B,D,F,H). Embryos carrying the p2x3b:egfp (A,B) or trpa1b:egfp (C,D) transgene were imaged at 72 hpf. Wild-type embryos were fixed at 72 hpf and in situ hybridization was performed for p2x3b (E,F) and trpa1b (G,H). Side view, anterior towards the left. Scale bars: 10 μm.