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Fig. 3 motionless embryos have reduced numbers of hypothalamic DA neurons and exhibit localized patterns of apoptosis. Lateral views (except C and D) of wildtype (left) and mot (right) embryos labeled with (A–F) anti-TH antibody, (G and H) anti-acetylated tubulin, or (I–L) TUNNEL stain. (A and B) 30-h embryos showing reduced hypothalamic DA neurons. (C and D) Ventral view showing a mild cyclopic brain, reduced hypothalamic DA neurons, and normal aac in 2-day-old mot embryos. (E and F) 2-day-old embryos showing few poorly differentiated hypothalamic THir cells and absent LC. (G and H) 30-h embryos showing that the major axonal tracts are grossly intact and the absence of brain ventricles. (I and J) 33-h mot embryos do not display excessive cell death compared to wildtype siblings. (K and L) 52-h embryos showing restricted cell death in the telencephalon and lens of the mot embryos. Abbreviations: aac, arch-associated CA cells; AC, anterior commisure; cb, cerebellum; Hy DA,hypothalamic dopaminergic neurons; LC, locus coeruleus; PC, posterior commisure; POC, postoptic commisure; t, telencephalon; te, tectum; tg, tegmentum; v, ventricle. Scale bar: 55 μm (A, B, I–L) and 110 μm (C, D, E, F, G, H).
Reprinted from Developmental Biology, 208, Guo, S., Wilson, S.W., Cooke, S., Chitnis, A.B., Driever, W., and Rosenthal, A., Mutations in the zebrafish unmask shared regulatory pathways controlling the development of catecholaminergic neurons, 473-487, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.