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Fig. S3 RNA polymerase II does not associate with mitotic chromosomes in pre-MBT or post-MBT embryos. Zebrafish embryos were fixed at the 256 cell and sphere stages. Mouse monoclonal anti-RNA polymerase II antibody (8WG16) was obtained from Covance. (A-A″ and B-B″) pre-MBT (256 cell), (C-C″) post-MBT (sphere). (A-C) DAPI staining, (A′-C′) anti-RNA polymerase II, (A″-C″) merged image. (A-A″) Interphase nuclei, (B-B″) mitotic chromosomes. Scale bar: 10 μm.
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