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Fig. 4 Comparison of array results with quantitative RT-PCR. Using the expression level of the 75% epiboly stage as reference, (A) the quantitative RT-PCR of β-actin (AF025305) at designated developmental stages have similar pattern to those obtained from the web resource which showed slightly increased expression after 24 hpf. The quantitative RT-PCR intensities for the selected (B) Node 2 gene (BM104515), (C) Node 3 gene (AW421995), (D) Node 4 gene (BG738899), and (E) Node 5 gene (AF254642) are closely correlated with the microarrary data.
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Reprinted from Gene expression patterns : GEP, 8(3), Ouyang, M., Garnett, A.T., Han, T.M., Hama, K., Lee, A., Deng, Y., Lee, N., Liu, H.Y., Amacher, S.L., Farber, S.A., and Ho, S.Y., A web based resource characterizing the zebrafish developmental profile of over 16,000 transcripts, 171-180, Copyright (2008) with permission from Elsevier. Full text @ Gene Expr. Patterns