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Fig. S3
Analysis of apoptosis in wild-type and morphant embryos
A-L Dorsal view of 3-days old zebrafish brains. A-C AnnexinV-Cy5 staining (B) in a APO-GFP (A) wild-type embryo. D-F AnnexinV-Cy5 staining (E) in a APO-GFP (D) morphant embryos. In both there is perfect colocalization between Annexin staining and the APO-E-GFP microglia. G-H Acridine Orange staining in wildtype (G) and a1-MO (H) embryos. I-J CaspGlow staining in wild-type (I) and a1- MO (J) embryos. K-L TMR-TUNEL staining in wild-type (K) and a1-MO (L) embryos.
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Reprinted from Cell, 133(5), Peri, F., and Nüsslein-Volhard, C., Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo, 916-927, Copyright (2008) with permission from Elsevier. Full text @ Cell