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Fig. S2 slow troponin C is expressed in all S58 positive slow fibres and in the pectoral fin muscle. (A) At 96 hpf slow troponin C (stnnC) is expressed in the slow fibres throughout the embryo and also in pectoral fin muscle. (B) At 96 hpf, slow troponin C ISH shows co-localisation with slow MyHC S58 antigen in the smyhc1:gfp-positive superficial slow fibres and in the sca muscle (dorsal view anterior trunk), in els and iob muscles (lateral view anterior trunk), and in scp and icp muscles (lateral view posterior tail). The pectoral fin muscle expresses slow troponin C but not slow MyHC S58 antigen. Scale bars: 25 μm. Abbreviations: els, embryonic lateralis superficialis; icp, infracarinalis posterior; iob, inferior obliquus; pf, pectoral fin; sca, supracarinalis anterior; scp, supracarinalis posterior.