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Fig. 5 Attenuation of Ndrg4 results in early looping abnormalities. The cmlc2-driven GFP is expressed in cardiomyocytes of transgenic Tg(cmlc2:gfp) embryos. At 2 dpf (A–D) and 3 dpf (E–H), compared to the clearly looped heart of uninjected Tg(cmlc2:gfp) embryos (A, C, E, G), the heart of ndrg4 morphant (B, D, F, H) remained central and linear in a tubular structure. (A, B, E, F), Lateral views with anterior towards the left; (C, D, G, H), frontal views with anterior towards the top. a, atrium; v, ventricle.
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Reprinted from Developmental Biology, 317(2), Qu, X., Jia, H., Garrity, D.M., Tompkins, K., Batts, L., Appel, B., Zhong, T.P., and Baldwin, H.S., ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish, 486-496, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.