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Fig. S3 Arrested pectoral fin bud induction in gsk3β morphants. At 72 hpf, wild-type pectoral fins elongate (A), but gsk3β morphants have still not developed fin buds (arrows; D). Whole mount in situ hybridization with shh and dlx2 staining reveal that the developed of fin bud were affected in gsk3β morphants. At 36 hpf, wild-type embryos continue shh (B) and dlx2 (C) expression in the developing bud mesenchyme, but in gsk3β morphants, the shh and dlx2 expression is greatly decreased.
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Full text @ BMC Dev. Biol.