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Fig. 8

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ZDB-IMAGE-080514-48
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Figures for Willot et al., 2002
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Fig. 8 Overexpression of admp or tr-admp RNA and in snailhouse. admp RNA (600 pg) or tr-admp RNA (600 pg) was injected at the one-cell stage into the progeny of snailhouse heterozygous fish. (A–C) Phenotypes were observed live after 1 day of development or (D–I) after in situ hybridization. (D–F) pax 2.1 and myo D in dark blue, hgg1 and krox 20 in red at the five- to seven-somite stage. (G–I) her5 and fkd6 in dark blue, hgg1, krox 20, and ntl in red at the six-somite stage. (A) Uninjected, -/+ or +/+ embryo. (B) Uninjected, snailhouse C4 phenotype. (C) admp-injected snailhouse mutant, C2 phenotype. (B, C) The eye is reduced in (C), compare with (B) (arrowhead). (D, G) Uninjected, -/+ or +/+ embryo. (E) Uninjected, snailhouse C4 phenotype. (F) admp-injected homozygous snailhouse embryo, C2 phenotype. (H) Uninjected, snailhouse C4 phenotype. The neural crest is expanded. (I) tr-admp-injected homozygous snailhouse embryo, C5 phenotype.

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Reprinted from Developmental Biology, 241(1), Willot, V., Mathieu, J., Lu, Y., Schmid, B., Sidi, S., Yan, Y.-L., Postlethwait, J.H., Mullins, M., Rosa, F., and Peyriéras, N., Cooperative action of ADMP- and BMP-mediated pathways in regulating cell fates in the zebrafish gastrula, 59-78, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.