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Fig. 1 Visualization of calcified skeletal structures in developing zebrafish embryos at 5, 6, 7, 9, 11, 14, 16, and 21 dpf by calcein staining. (A, B) Ventral view of the head skeleton of day-7 (A) and -11 (B) embryos labeled with calcein. m, Meckel’s cartilage; pq, palatoquadrate; e, ethmoid plate. (C, D) Side view of the head skeleton of day-7 (A) and -11 (B) embryos labeled with calcein. n, notochord; c, cleitrum.
                
                    
                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
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    Reprinted from Developmental Biology, 238(2), Du, S., Frenkel, V., Kindschi, G., and Zohar, Y., Visualizing normal and defective bone development in zebrafish embryos using the fluorescent chromophore calcein, 239-246, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.
 
        