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Fig. 1

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ZDB-IMAGE-080502-1
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Figures for Kreiling et al., 2008
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Fig. 1 Cytosolic Ca2+ patterns in gastrula stage embryos. (A–C) Ratiometric imaging of cytosolic Ca2+ patterns at 6 hpf during normal development. (D–F) Cytosolic Ca2+ patterns at 6 hpf following inhibition of the ER Ca2+ pump with 0.5 μM thapsigargin from 4 to 6 hpf. Ca2+ concentrations are elevated in all embryonic regions, including the ventral margin (v), dorsal margin (d), and dorsal forerunner cells (DFC). (A and D) Overlay of bright field (grey-scale), the calcium indicator Oregon Green BAPTA-1 dextran (green), and the calcium-insensitive control Texas Red dextran (red). (B and E) Overlay of Oregon Green BAPTA-1 dextran and Texas Red dextran. (C and F) Pseudocolored ratiometric images (green/red). (G) The bar graph shows average cytosolic Ca2+ concentrations in the ventral margin (V), dorsal margin (D), and dorsal forerunner cells (DFC). The average Ca2+ concentration was measured in a circular region of interest with a diameter of 40 μm (ventral and dorsal margin) or 20 μm (dorsal forerunner cells). In the DMSO-treated controls, Ca2+ concentrations are significantly lower in the dorsal margin and DFC compared to the ventral margin (two-tailed t-test, P < 0.05, n = 13). Ca2+ concentrations increase significantly in the ventral margin, dorsal margin, and dorsal forerunner cells following treatment with 0.5 μM thapsigargin from 4 to 6 hpf (two-tailed t-test, P < 0.05, n = 13). Scale bar = 200 μm.

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Reprinted from Mechanisms of Development, 125(5-6), Kreiling, J.A., Balantac, Z.L., Crawford, A.R., Ren, Y., Toure, J., Zchut, S., Kochilas, L., and Creton, R., Suppression of the endoplasmic reticulum calcium pump during zebrafish gastrulation affects left-right asymmetry of the heart and brain, 396-410, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.