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Fig. 1 In situ hybridization (probed with FITC-oligo γ-tubulin probe) of different stages of zebrafish oocytes (A and G are fully grown, immature oocytes; B and H are mature oocytes or eggs) and embryos (C and I are 32 cell; D and J are 256 cell; E and K are ∼30% epiboly gastrula; F and L are pharyngula). The top panels are “with probe” treatments (A–F) and the lower panels are corresponding “without probe” treatments (G–L). The primordial blastodisc (b) that forms at the animal pole of the egg during oocyte meiotic maturation labels intensely with the probe (B). Inset: immature oocyte probed after hemisection showing cortical label. Anti-FITC second antibody conjugated to alkaline phosphatase and the substrate DAB were used to develop color. The specimens were dehydrated in 100% MeOH and placed in clearing media benzyl benzoate:benzyl alcohol (2:1) prior to mounting on slides. Scale bar equals 250 μm.
Reprinted from Gene expression patterns : GEP, 8(4), Liu, J., and Lessman, C.A., Changes of gamma-tubulin expression and distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo, 237-247, Copyright (2008) with permission from Elsevier. Full text @ Gene Expr. Patterns