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Fig. 7 Pax 6 mRNA expression is disrupted in OM2 morphants. (A, C, E and G) Pax6.1 in situ hybridization of 28–54 hpf control fish eyes. (B, D, F and H) Pax6.1 in situ hybridization of 3i4e MO-injected fish eyes. Notice the reduction of Pax6.1 mRNA level in the eye at all ages, but Pax6.1 mRNA can still be observed in the brain. Arrows in (E) and (G) indicates a sharply delineated Pax6.1 mRNA expression pattern at 54 hpf, which was lost in OM2 morphant eyes (F and H). (I, K, M and O) Pax6.2 in situ hybridization of 25–54 hpf control fish. High level of Pax6.2 mRNA expression is particularly evident in the 54 hpf eye with sharp, circular boundaries (arrows in M and O). (J, L, N and P) Pax6.2 in situ hybridization of 25–54 hpf 3i4e MO-injected fish. Much weaker Pax6.2 mRNA expression with less well-defined layers is observed in the eye at all developmental stages.
Reprinted from Mechanisms of Development, 125(1-2), Lee, J.A., Anholt, R.R., and Cole, G.J., Olfactomedin-2 mediates development of the anterior central nervous system and head structures in zebrafish, 167-181, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.