Fig. 3

Fig. 3 Redundant mechanisms involved in nok nuclear accumulation. Images are reconstructions of confocal Z-stack sections imaged on late gastrula stage whole mounts. HisMyc-tagged nok deletion proteins are detected with anti-Myc antibody (green). A: HisMyc-tagged nok^ΔPatj strongly localizes to nuclei of EVL and DL cells (recognizable by their size) and to outer cell membranes. B: HisMyc-tagged nok^{ΔPatj NLS} protein that lacks the L27N domain and the two nuclear import signals still localizes to the nucleus, which indicates that alternatives routes of nok nuclear import must exist. C: HisMyc-tagged nok nok^{ΔPar6 Patj} protein localizes to nuclei of EVL and DL cells indicating that direct association with Par6 is not necessary for nuclear accumulation of nok. D: In contrast, HisMyc-tagged nok^{ΔPar6 Patj NLS} protein fails to localize to nuclei of EVL and DL cells suggesting redundancy of the NLS motifs and association with Par6 in nok nuclear accumulation. E: Control non-injected embryos stained with the anti-Myc antibody.