Manipulation of EphB. (A, B) Incubation of a brain in unclustered Ephrin-B2/Fc did not prevent habenular commissure formation (arrowhead). The ligand did bind to commissural axons, as indicated by Alexa 546 anti-Fc antibody (A). (B) Acetylated tubulin labeling, showing axon tracts. (C) An axon expressing truncated EphB3, fused to GFP (arrow). The axon is traversing the roof plate.
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