ZFIN Image: Lachnit et al., 2008, Fig. S4

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Figure Caption

Fig. S4 Zygotic Pou5f1 impact on DEL and EVL vegetal closure progression. (A–B) Z-projection of confocal image stacks of a vegetal view of embryos at approximately 90% epiboly stage stained with Sytox green to visualize nuclei. (A) Wild-type (B) Zspg^m793. (C) Measurement of the distance of opposing DEL and EVL margins. DEL cells of Zspg^m793 displayed an average distance of 341 ± 31 μm and those of wild-type siblings 307 ± 31 μm (p < 0.160) The distance of opposing EVL margins was 220 ± 40 μm in wild-type and 217 ± 19 μm in Zspg^m793 (p < 0.951). Embryos resulted from the cross female spg/+x male spg/spg or from the cross female spg/+x male spg/+. The genotypes of embryos were identified after confocal data acquisition by PCR. Scale bar: 100 μm.

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Acknowledgments

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Reprinted from Developmental Biology, 315(1), Lachnit, M., Kur, E., and Driever, W., Alterations of the cytoskeleton in all three embryonic lineages contribute to the epiboly defect of Pou5f1/Oct4 deficient MZspg zebrafish embryos, 1-17, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.