|
Fig. 5 Induction of hsp27 and hsp27-gfp expression by arsenic. (A,B) lateral (A) and ventral (B) view of 6 dpf untreated control hsp27-gfp fry. (C,D) Lateral (C) and ventral (D) view of 6 dpf fry treated with arsenic. hsp27-gfp fry were treated with 100 ppm sodium arsenate for 48 h and allowed to recover for 24 h before GFP fluorescence observation. Images of control and treated embryos were taken with the same camera exposure. (E–J) Whole-mount in situ analysis of hsp27 mRNA expression in 5 dpf fry without arsenic treatment (E, F), or treated with 50 ppm (G, H) and 200 ppm (I, J) sodium arsenate. The arsenic treatment was carried out at 72 hpf for 48 h prior to fixation for in situ hybridization. Panels E, G and I are lateral view; panels F, H and J ventral view. Abbreviation: h, heart; hh, hyohyoideus; ih, interhyal; ima, intermadibularis anterior; imp, intermendibularis posterior; mhb, midbrain hindbrain boundary; som, somite. The nomenclature for cranial skeletal muscles is based on Schilling and Kimmel (1997). Scale bar: 100 μm.
Reprinted from Gene, 408(1-2), Wu, Y.L., Pan, X., Mudumana, S.P., Wang, H., Kee, P.W., and Gong, Z., Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter, 85-94, Copyright (2008) with permission from Elsevier. Full text @ Gene