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Fig. 1 hai1a is expressed in basal keratinocytes and is required for the proper development of the epidermis. (A,B) Lateral views of the trunk of a wild-type sibling (WT; A) and a homozygous hi2217 embryo (hai1a-/-; B) at 48 hpf, after anti-p63 immunolabeling of basal keratinocytes. (C) Shedding of cells into the chorion is evident in hi2217 homozygotes (right embryo), compared with no shedding in the wild-type sibling (left embryo) from 24 hpf. (D,E,H) In situ hybridization showing the expression of hai1a at 24 hpf (D) and 48 hpf (E), and of hai1b at 24 hpf (H). The hai1a and hai1b expression domains in olfactory epithelium (olf), otic epithelium (ot), gut (gt), lateral line primordium (llp), neuromasts (nm) and pharyngeal endoderm (pe) are indicated. (F,G,I) Cross-section through the posterior trunk of a 24 hpf wild-type embryo after double staining for hai1a mRNA (blue) and p63 protein (brown) (F), and magnified views of the trunk epidermis of a 24 hpf wild-type embryo after double staining for hai1a (G) or hai1b (I) (blue) and p63 protein (brown). In F, the hai1a expression domains in the pronephric ducts (pn) and gut (gt) are indicated.