Fig. 6 hhex does not repress wnt8 expression at the dorsal margin. (A,B) Whole-mount in situ hybridization for hhex in a cycb16 mutant embryo at 50% epiboly. cycb16 is a deletion covering the hhex locus, such that mutant embryos lack hhex expression. (C,D) Expression of wnt8 in a wild-type embryo. (E,F) Expression of wnt8 in a representative embryo from a cycb16+/- intercross at shield stage: wt, deficiency heterozygous and cycb16 mutant embryos have indistinguishable wnt8 expression. (A,C,E: animal view, in C and E dorsal to the right; B,D,F: lateral view, in D and F dorsal to the front; probes used are indicated in the bottom left corner of each panel).
Reprinted from Developmental Biology, 276(2), Bischof, J., and Driever, W., Regulation of hhex expression in the yolk syncytial layer, the potential Nieuwkoop center homolog in zebrafish, 552-562, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.