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Fig. S7 Effect of Tob1a on Biochemical Properties of Smad3 (A) Formation of Flag-Smad3/HA-caALK4 complex (indicated by an arrow) was not affected by coexpression of tob1a (1 μg and 4 μg from left to right) in HEK293T cells. (B) Formation of Flag-Smad3/HA-Smad4 complex was not affected by coexpression of tob1a (1 μg and 4 μg from left to right) in HEK293T cells. (C) The level of phosphorylated Smad3 (indicated by an arrow), which was promoted by coexpression of caALK4, was not affected by coexpression of tob1a (1 μg and 4 μg from left to right) in HEK293T cells.
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Reprinted from Developmental Cell, 11(2), Xiong, B., Rui, Y., Zhang, M., Shi, K., Jia, S., Tian, T., Yin, K., Huang, H., Lin, S., Zhao, X., Chen, Y., Chen, Y.G., Lin, S.C., and Meng, A., Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity, 225-238, Copyright (2006) with permission from Elsevier. Full text @ Dev. Cell