ZFIN Image: Lakowski et al., 2007, Fig. S2

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Figure Caption

Fig. S2 EGFP expression typically seen in zebrafish embryos transiently transgenic for the dualreporter BAC at 1 dpf. (A) These eight embryos were randomly chosen from a group of 80 embryos. (B) In this group, seven embryos exhibited comparable EGFP fluorescence, and one had only a few EGFP-expressing cells in the eye (*). In this group, 74 embryos (>90%) exhibited EGFP expression. None of these embryos exhibited EGFP expression in cells outside of the normal domain of Pax6a expression. (C) PCR genotyping analysis indicates that >90% (n=94/100 embryos; three independent trials) of the injected embryos retain the BAC transgene after 1 day of development. Eight representative examples are shown: six are from embryos injected with the BAC transgene (1-6), and two are from uninjected controls (-). +, positive control.

Acknowledgments

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Reprinted from Developmental Biology, 307(2), Lakowski, J., Majumder, A., and Lauderdale, J.D., Mechanisms controlling Pax6 isoform expression in the retina have been conserved between teleosts and mammals, 498-520, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.