PUBLICATION
Mechanisms controlling Pax6 isoform expression in the retina have been conserved between teleosts and mammals
- Authors
- Lakowski, J., Majumder, A., and Lauderdale, J.D.
- ID
- ZDB-PUB-070523-20
- Date
- 2007
- Source
- Developmental Biology 307(2): 498-520 (Journal)
- Registered Authors
- Lauderdale, James D., Majumder, Anirban
- Keywords
- Transgene, Alternative promoter, Retina, Eye
- MeSH Terms
-
- Promoter Regions, Genetic
- Animals
- Repressor Proteins/genetics*
- Neuroglia/metabolism
- Recombinant Proteins/genetics
- Retinal Bipolar Cells/metabolism
- Mice, Transgenic
- Sequence Homology, Nucleic Acid
- Gene Expression Regulation, Developmental
- Evolution, Molecular
- Genes, Reporter
- Mammals/genetics
- Paired Box Transcription Factors/genetics*
- Molecular Sequence Data
- Base Sequence
- Zebrafish/embryology*
- Zebrafish/genetics*
- Chromosomes, Artificial, Bacterial/genetics
- Protein Isoforms/genetics
- DNA Primers/genetics
- Animals, Genetically Modified
- Conserved Sequence
- Retina/cytology
- Retina/embryology*
- Retina/growth & development
- Retina/metabolism*
- Homeodomain Proteins/genetics*
- Luminescent Proteins/genetics
- Zebrafish Proteins/genetics*
- Eye Proteins/genetics*
- Amacrine Cells/metabolism
- Green Fluorescent Proteins/genetics
- Mice
- PubMed
- 17509554 Full text @ Dev. Biol.
Citation
Lakowski, J., Majumder, A., and Lauderdale, J.D. (2007) Mechanisms controlling Pax6 isoform expression in the retina have been conserved between teleosts and mammals. Developmental Biology. 307(2):498-520.
Abstract
The Pax6 gene plays several roles in retinal development, including control of cell proliferation, maintenance of the retinogenic potential of progenitor cells, and cell fate specification. Emerging evidence suggests that these different aspects of Pax6 gene function are mediated by different isoforms of the Pax6 protein; however, relatively little is known about the spatiotemporal expression of Pax6 isoforms in the vertebrate retina. Using bacterial artificial chromosome (BAC) technology, we modified a zebrafish Pax6a BAC such that we could distinguish paired-containing Pax6a transcripts from paired-less Pax6a transcripts. In the zebrafish, the spatial and temporal onset of expression of these transcripts suggests that the paired-less isoform is involved in the cell fate decision leading to the generation of amacrine cells; however, because of limitations associated with transient transgenic analysis, it was not feasible to establish whether this promoter was active in all amacrine cells or in a specific population of amacrine cells. By making mice transgenic for the zebrafish Pax6a BAC reporter transgene, we were able to show that paired-containing and paired-less Pax6a transcripts were differentially expressed in amacrine subpopulations. Our study also directly demonstrates the functional conservation of the regulatory mechanisms governing Pax6 transcription in teleosts and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping