COX-1 knockdown results in gastrulation defect at high dose. Injection of 10 ng cox1-MO results in gastrulation arrest at ~50% epiboly stage (A). Gastrulation arrest defect by cox1-MO can be rescued by co-injecting synthetic cox1 RNA, mutated in MO-binding site (B). Black bar indicates the percentage of embryos that survived to tailbud (C). In the rescue experiments (lane 3, 4), we tested at least 80 embryos from two independent experiments, while in cox1 RNA injection groups (lane 5, 6), at least 50 embryos from two independent experiments were analyzed.
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