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Fig. 2 Stereomicroscopic observation of Brul expression after whole-mount immunostaining. In each panel the embryo on the left was immunoreacted with anti-Brul antiserum, and that on the right was reacted with preimmune serum as negative control. Brul is distributed maternally (A–C), accumulates at the animal pole during the 1-cell stage (D–F), and exists over the whole embryos throughout embryonic development (G–K). During early cleavage stages, specific accumulation was observed at the ends of the cleavage furrows (arrowheads in G), whereas Brul was distributed evenly in the blastomeres (G). Fluorescent intensity was reduced in the shield and 3-somite stages. Brul was also detected in unfertilized embryos (L). A–F and L are lateral views with the animal pole up; G and H are lateral oblique views; I is an animal pole view with dorsal side left; J and K are lateral views.
Reprinted from Gene expression patterns : GEP, 6(2), Hashimoto, Y., Suzuki, H., Kageyama, Y., Yasuda, K., and Inoue, K., Bruno-like protein is localized to zebrafish germ plasm during the early cleavage stages, 201-205, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns