Fig. 2 tortuga regulates her1 at the post-transcriptional level. Embryos were hybridized with an in situ hybridization probe that binds the third intron of her1 and thus detects nascent and/or unprocessed transcript. At the 3 somite stage, mutant embryos in a clutch derived from tor+/- parents cannot be distinguished from wild type siblings (A). At 12 and 18 somite stages, tor mutant embryos were sorted from their wild type siblings by morphological criteria before hybridization; again, no differences in the pattern of her1 transcription in wild type and tor mutant embryos are observed (B–E). Panels are dorsal views, anterior top.
Reprinted from Developmental Biology, 287(2), Dill, K.K., and Amacher, S.L., tortuga refines Notch pathway gene expression in the zebrafish presomitic mesoderm at the post-transcriptional level, 225-236, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.