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Fig. S1 Additional wt1a and wt1b morpholinos serve as controls for specificity. Embryos injected either with control morpholino, a morpholino blocking translation of the wt1a mRNA (AUG) or a morpholino targeting the second splice donor site of the wt1b pre-mRNA (Splc2-2) are shown. Note that the phenotypes resemble closely those of embryos, which were injected with a morpholino targeting the first splice donor site of the respective gene (Fig. 1C).
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Reprinted from Developmental Biology, 309(1), Perner, B., Englert, C., and Bollig, F., The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros, 87-96, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.