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Fig. S3 Characterization of pSUPER-Aida. About 0.3 μg of HA-Aida expression plasmid was co-transfected with 8 μg of pSUPER vector, control RNAi or pSUPER-Aida into HEK 293T cells; pEGFP was co-transfected as internal transfection control. At 30 h posttransfection, cells were lysed and immunoblotting was carried out with anti-HA antibody to compare the Aida protein levels int eh presence or absence of pSUPER-Aida
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Reprinted from Developmental Cell, 13(2), Rui, Y., Xu, Z., Xiong, B., Cao, Y., Lin, S., Zhang, M., Chan, S.C., Luo, W., Han, Y., Lu, Z., Ye, Z., Zhou, H.M., Han, J., Meng, A., and Lin, S.C., A beta-Catenin-Independent Dorsalization Pathway Activated by Axin/JNK Signaling and Antagonized by Aida, 268-282, Copyright (2007) with permission from Elsevier. Full text @ Dev. Cell