ZFIN ID: ZDB-PUB-070813-12
A beta-Catenin-Independent Dorsalization Pathway Activated by Axin/JNK Signaling and Antagonized by Aida
Rui, Y., Xu, Z., Xiong, B., Cao, Y., Lin, S., Zhang, M., Chan, S.C., Luo, W., Han, Y., Lu, Z., Ye, Z., Zhou, H.M., Han, J., Meng, A., and Lin, S.C.
Date: 2007
Source: Developmental Cell   13(2): 268-282 (Journal)
Registered Authors: Meng, Anming
Keywords: none
MeSH Terms:
  • Animals
  • Axin Protein
  • Body Patterning*/drug effects
  • COS Cells
  • Carrier Proteins/metabolism*
  • Cell Line
  • Chlorocebus aethiops
  • Dimerization
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/enzymology
  • Enzyme Activation/drug effects
  • Humans
  • JNK Mitogen-Activated Protein Kinases/metabolism*
  • MAP Kinase Signaling System*/drug effects
  • Mice
  • Oligonucleotides, Antisense/pharmacology
  • Phenotype
  • Protein Binding/drug effects
  • Repressor Proteins/chemistry
  • Repressor Proteins/metabolism*
  • Wnt Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism*
  • beta Catenin/metabolism*
PubMed: 17681137 Full text @ Dev. Cell
Axin is a scaffold protein that controls multiple important pathways, including the canonical Wnt pathway and JNK signaling. Here we have identified an Axin-interacting protein, Aida, which blocks Axin-mediated JNK activation by disrupting Axin homodimerization. During investigation of in vivo functions of Axin/JNK signaling and aida in development, it was found that Axin, besides ventralizing activity by facilitating beta-catenin degradation, possesses a dorsalizing activity that is mediated by Axin-induced JNK activation. This dorsalizing activity is repressed when aida is overexpressed in zebrafish embryos. Whereas Aida-MO injection leads to dorsalized embryos, JNK-MO and MKK4-MO can ventralize embryos. The anti-dorsalization activity of aida is conferred by its ability to block Axin-mediated JNK activity. We further demonstrate that dorsoventral patterning regulated by Axin/JNK signaling is independent of maternal or zygotic Wnt signaling. We have thus identified a dorsalization pathway that is exerted by Axin/JNK signaling and its inhibitor Aida during vertebrate embryogenesis.