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Fig. 2 Temporal and spatial distributions of bmp1 expression. (A) RT-PCR analysis of bmp1 and mfn/tll1 RNA levels at different stages of zebrafish development. Expression of tubulin is shown as a control for loading. Each experiment was performed three times with similar results. Spatial bmp1 expression patterns at different stages. (B) Ninety percent epiboly, lateral view, dorsal is to the right. Arrow, bmp1 signal in the ventral quadrant of the animal pole; Arrowhead, signal at the ventral margin. (C) Bud stage, lateral view, dorsal is to the right. TB and arrow denote bmp1 signal in the tail bud. (D) Fifteen somite stage, lateral view, anterior is to the left. Arrows show bmp1 signal in dorsal diencephalon, future tectum, caudal somites/tail bud. (E) Twenty-four hours post fertilization embryo, lateral view, anterior is to the left. Bmp1 signal is detected in pectoral fin bud (FB), Epiphysis (Ep), dorsal diencephalon (DD), and caudal somites/tail bud (TB). (F) Twenty-four hours post fertilization embryo brain, dorsal view, showing bmp1 mRNA localization to the Zona Limitans Interthalamica (ZLI).
Reprinted from Mechanisms of Development, 123(7), Jasuja, R., Voss, N., Ge, G., Hoffman, G.G., Lyman-Gingerich, J., Pelegri, F., and Greenspan, D.S., bmp1 and mini fin are functionally redundant in regulating formation of the zebrafish dorsoventral axis, 548-558, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.