ZFIN Image: Horsfield et al., 2007, Fig. S3

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Figure Caption

Fig. S3 gata1 expression levels are reduced in rad21 morphants and mutants during early embryogenesis. Whole-mount embryos stained for gata1 expression. Upper panels are wild type; lower panels are rad21ATGMO morphants (MO) or rad21nz171 homozygotes as indicated. (A) Posterior views of embryos, stages as indicated. Onset of expression of gata1 is slightly delayed in rad21 morphants, as evidenced by gata1 expression in the 6s morphant appearing similar to its expression in the 4s wild-type embryo. All rad21 morpholino-injected embryos (rad21ATGMO; 0.5 pmol) at 4s (n=29/29) and 6s (n=20/20) had reduced/delayed expression of gata1. On close inspection of rad21nz171 mutants, gata1 expression appears to be ‘patchy’ in intensity within the PLM stripes. (B) Lateral views, anterior to the left, stages as indicated. gata1 expression is noticeably reduced in the PLM/ICM of rad21nz171 mutants.

Figure Data

Expression / Labeling details

Acknowledgments

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