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Fig. 3 sfpq expression patterns. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) for sfpq in embryonic tissue shows that sfpq is expressed beginning at 1 hpf (lane 1) and peaks at 18 hours postfertilization (hpf, lane 5). Adult tissue also has sfpq expression, with the brain showing the highest level (lane 11) and heart and body muscle showing lower levels (lanes 14, 15). Actin RT-PCR was used as control for RNA levels. B-Q: sfpq in situ hybridization time course shows that sfpq is expressed throughout embryogenesis. B: At 1 hpf (four-cell stage), side view. C: At 4 hpf (blastula stage), side view. D: At 8 hpf (75% epiboly stage), side view, dorsal right. E: At 12 hpf (six-somite stage), side view, dorsal right, anterior top. F-H: At 18 hpf, strongest expression in the forebrain (H, bracket) and in presumptive rhombomere 4 (H, arrow). I-K: At 24 hpf. L-N: At 2 dpf, expression appears restricted to strong longitudinal strips in the ventral brain (M, arrow) and in weaker transverse stripes in the hindbrain. O-Q: At 3 dpf, expression ventral to brain (P, arrow) and along axon tracts. F-G,I-J,L-M,O-P: Side views, anterior left. H,K,N,Q: Dorsal views, anterior left. Note: in I-Q, regions without staining may not be in focus, as to allow high magnification imaging of the stained regions. In areas of staining, the fuzziness sometimes observed is due to low levels of diffuse staining, not poor imaging. Scale bar = 100 μm.