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Fig. 2 Csk knockdown induced defects associated with impaired gastrulation. Zebrafish embryos were not injected (A) or microinjected with Fyn/Yes-MO (5 ng) (B) or Csk-MO (5 ng) (C) at the 1 cell stage and allowed to develop. (A–C) Morphology at 10 hpf shows reduced anterior extension of the Fyn/Yes-MO and Csk-MO injected embryos. (D) Csk knock down embryos at 3 dpf show a reduction in overall length (uninjected at the top). (E) Rescue of the Csk knock down phenotype by co-injection of synthetic human csk mRNA. Zebrafish embryos were injected with Csk-MO (5 ng) alone or in conjunction with human csk mRNA (150 pg). The length of the embryos was measured at 3 dpf and the average is shown here. Two tailed student t-tests indicate a significant decrease in length after injection of Csk-MO alone (P < 0.001, single asterisk) and a significant increase in the length after co-injection of csk mRNA (P < 0.001, double asterisk).
Reprinted from Mechanisms of Development, 124(2), Jopling, C., and den Hertog, J., Essential role for Csk upstream of Fyn and Yes in zebrafish gastrulation, 129-136, Copyright (2007) with permission from Elsevier. Full text @ Mech. Dev.