ZFIN Image: Schäfer et al., 2005, Fig. 4

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Figure Caption

Fig. 4

Ectopic shh, but not cyc expression results in dorsal expansion of nkx2.2b. (A-F) Expression of nkx2.2b in 18s wild-type embryos (A-C) and embryos injected with 2 pg cyc RNA (D-F). Note enlargement of axial structures (notochord, MFP) evident in transversal section in (F), but only mildly affected nkx2.2b expression in head (D) and trunk (E,F). (G-I) Expression of nkx2.2b in oep-/- mutants. Note missing MFP (I), but normal nkx2.2b expression in head (G) and trunk (H,I). (J-L) nkx2.2b expression in embryos injected with 80 pg shh RNA. nkx2.2b shows strong dorsal expansion in distinct regions of the brain and the complete spinal cord. (A,D,G,J) are lateral views of the head and (B,E,H,K) of the trunk, anterior to the left. (C,F,I,L) are transversal sections through the neural tube at trunk levels. Scale bar in (J) represents 100 µm (for A,D,G,J), in (K) 50 µm (for B,E,H,K), and in (L) 20 µm (for C,F,I,L).

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Acknowledgments

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Reprinted from Mechanisms of Development, 122(1), Schäfer, M., Kinzel, D., Neuner, C., Schartl, M., Volff, J.N., and Winkler, C., Hedgehog and retinoid signalling confines nkx2.2b expression to the lateral floor plate of the zebrafish trunk, 43-56, Copyright (2005) with permission from Elsevier. Full text @ Mech. Dev.