Fig. 4
Fig. 4 Sequence, phylogeny, and RT-PCR of zebrafish RXRs. A: Sequence alignment of zebrafish RXRs. Alignment was performed with ClustalW. B: Phylogenetic analysis of all available full-length sequences. Another nuclear receptor, human PPAR, was defined as the outgroup. Phylogeny was constructed using the neighbor-joining method with PAUP*. Numbers on the nodes reflect bootstraps from 1,000 replicates. C: RT-PCR for the zebrafish rxr genes from cDNA collected from 25 2-8 cell or 15-20 somite embryos. -RT control indicated lack of genomic DNA contamination (not shown). Arrow indicates low maternal expression of rxraa. Asterisk indicates the newly identified gene rxraa. Carrot indicates the newly identified gene rxrgb.